Introduction to Fluorescence Sensing

Introduction to Fluorescence Sensing

Fluorescence sensing is a rapidly developing field of research and technology. Its target is nearly the whole world of natural and synthetic compounds being detected in different media including living bodies. The application area range from control of industrial processes to environment monitoring and clinical diagnostics. Among different detection methods fluorescence techniques are distinguished by ultimate sensitivity, high temporal and spatial resolution and versatility that allows not only remote detection of different targets but their imaging within the living cells. The basic mechanism of sensing is the transmission of the signal produced by molecular interaction with the target to fluorescent molecules, nanoparticles and nanocomposites with the detection by devices based on modern electronics and optics. In this interdisciplinary field of research and development the book is primarily intended to be a guide for students and young researchers. It is also addressed to professionals involved in active research and product development serving as a reference for the recent achievements. The users of these products will find description of principles that could allow proper selection of sensors for particular needs. Making a strong link between education, research and product development, this book discusses future directions.

Introduction to Fluorescence Sensing

Molecular Imaging of the Brain

Molecular Imaging of the Brain

This book examines multi-quantum magnetic resonance imaging methods and the diagnostics of brain disorders. It consists of two Parts. The part I is initially devoted towards the basic concepts of the conventional single quantum MRI techniques. It is supplemented by the basic knowledge required to understand multi-quantum MRI. Practical illustrations are included both on recent developments in conventional MRI and the MQ-MRI. This is to illustrate the connection between theoretical concepts and their scope in the clinical applications. The Part II initially sets out the basic details about quadrupole charge distribution present in certain nuclei and their importance about the functions they perform in our brain. Some simplified final mathematical expressions are included to illustrate facts about the basic concepts of the quantum level interactions between magnetic dipole and the electric quadrupole behavior of useful nuclei present in the brain. Selected practical illustrations, from research and clinical practices are included to illustrate the newly emerging ideas and techniques. The reader should note that the two parts of the book are written with no interdependence. One can read them quite independently.

Molecular Imaging of the Brain

Atoms in Plasmas

Atoms in Plasmas

A study of radiative-collisional phenomena in neutral and ionized gases, focusing on a ‘perturbed atom’, i.e an atom under the influence of different perturbations in plasmas, namely by electrical and magnetic fields. The treatment covers fundamental aspects of modern physics, such as atomic quantum mechanics and quantum optics, radiation and collisional processes in plasmas and gases, nonlinear laser spectroscopy, and plasma diagnostics.

Atoms in Plasmas

Mortalin Biology

Mortalin Biology

The phrase Life, stress and death connects three terms, but is there a biological basis for that? Are there molecules that are essential to/or mediate these phenomena? This contributory volume Mortalin Biology: Life, Stress and Death is a remarkable compilation of the research outcomes on the stress protein mortalin, a member of heat shock 70 family of proteins. The book is unique as it describes mortalin playing essential role in life, stress response and death either from cancer, when it becomes hyperactive or from neuro-degeneration, when it becomes hypoactive. The book provides up-to-date knowledge on mortalin with respect to its discovery, structure, evolutionary conservation, function and signal transduction in different organisms in a simple, but most comprehensive way, that besides offering an enjoyable and in-depth reading, prompts the reader to ask further questions to explore this protein with new ideas, approaches and experiments. Twenty-one chapters by the world leaders on the specific areas of mortalin research throw light on its multi-functionality, potentials for biotechnology, diagnostics and therapeutic values. Avenues of mortalin biology, yet unexplored, hold immense promises for future, and reading this volume provides an easy, enthusiastic and energetic head-on start.

Mortalin Biology

Mechanics of Biological Tissue

Mechanics of Biological Tissue

The mechanics of biological tissues is a multidisciplinary and rapidly expanding area of research. The book points to important directions combining mechanical sciences with the exciting new developments in biology. It delivers state-of-the-art articles on: Mechanics of tissues at the molecular, cellular, tissue and organ levels. Mechanobiology, with particular reference to growth, remodeling, repair and aging. Experimental, microstructural and continuum mechanical perspectives with an emphasis on modelling, and simulating therapeutic and diagnostic procedures. Implementation of mechanical models in numerical codes to provide a tool for the design and development of prostheses. Numerical models have the potential to greatly improve diagnostics and therapeutical procedures that involve tissue mechanics.

Mechanics of Biological Tissue

Surface Chemistry Essentials

Surface Chemistry Essentials

Surface chemistry plays an important role in everyday life, as the basis for many phenomena as well as technological applications. Common examples range from soap bubbles, foam, and raindrops to cosmetics, paint, adhesives, and pharmaceuticals. Additional areas that rely on surface chemistry include modern nanotechnology, medical diagnostics, and drug delivery. There is extensive literature on this subject, but most chemistry books only devote one or two chapters to it. Surface Chemistry Essentials fills a need for a reference that brings together the fundamental aspects of surface chemistry with up-to-date references and data from real-world examples.

Surface Chemistry Essentials

Smart Sensors and Mems

Smart Sensors and Mems

Smart sensors and micro electro mechanical systems (MEMS) can include a variety of devices and systems that have a high level of functionality. They may integrate sensing and actuating with information processing, analog to digital conversion and memory functions. Smart sensors and MEMS may respond to algorithmic instructions and calibration requests, allowing smart devices to become more autonomous as they provide self diagnostics and self correction. Intelligence enables adaptation, which in turn increases the reliability and longevity of the smart device and this can significantly reduce costs. The editors have brought together a roster of contributing experts to cover smart sensors including capacitive, inductive, resistive and image sensors, smart MEMS including power MEMS, optical MEMS, inertial MEMS, and MEMS print heads.

Smart Sensors and Mems

Single Cell Diagnostics

Single Cell Diagnostics

Single Cell Diagnostics: Methods and Protocols applies modern molecular diagnostic techniques to the analysis of single cells, small numbers of cells, or cell extracts. Emphasis is placed on non-invasive analysis of single cell metabolites and the direct analysis of RNA and DNA from single cells, with a focus on polymerase chain reaction and fluorescence in situ hybridization. The authors, all leading scientists in the field, provide current and innovative methods for extracting useful, accurate, and reliable data from single-cell samples. The stepwise, technically precise protocols presented in this volume thoroughly describe single-cell assays, from material collection and isolation of cells to data interpretation. Cell-types analyzed include embryonic blastomeres, fibroblasts, and lymphocytes. Among the techniques described are real-time quantitative PCR, isothermal whole genome amplification, comparative genomic hybridization, real-time genetic expression analysis, and the production of RNA and cDNA libraries. This handbook will be essential for researchers and clinicians, and practitioners providing care for couples seeking treatment for infertility or preimplantation genetic diagnosis. Book jacket.

Single Cell Diagnostics